Quantitative polymerase chain reaction to estimate the number of amplified esterase genes in insecticide-resistant mosquitoes.

ogy as with DSPE–PEG 2000. Since the concentrations of PEG 5000 containing proteins could also be measured by Skoog’s method (13), we believe that this modified method would also work. In conclusion, a simple and direct colorimetric method, modified from Skoog’s PEG measurement method (13), for determining the amount of DSPE– PEG 2000 in buffer solutions containing blood or albumin was reported. The concentrations of DSPE–PEG 2000 in those solutions could be determined at 435 nm as low as 25 mg/ml with simple sample preparations. It may facilitate the application of PEG–liposomes in drug delivery or blood substitutes.